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Biotium Renilla Luciferase Assay Kit 2.0, 150 Assays
Supplier: Biotium 300821
This kit is designed to measure Renilla luciferase activity in transfected cell reporter gene assays. Renilla luciferase has been used as a reporter gene for studying gene regulation and function in vitro and in vivo. The enzyme is a a monomeric 36,000 Dalton protein that catalyzes coelenterazine oxidation by oxygen to produce light. Coelenterazine also emits light from enzyme-independent oxidation, a process known as autoluminescence. The autoluminescence is enhanced by superoxide anion and peroxynitrite in cells and tissues. This assay kit utilizes a special buffer formulation designed to yield reliable, linear measurements of Renilla luciferase activity with minimal autoluminescence background and superior sensitivity. The kit is suitable for plate sizes ranging from 96-well to 24-well and has reagents sufficient for 150 assays.
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