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AdipoGen Resorufin-isobutyrate

Supplier: AdipoGen CDXI0005M005
Chemical. CAS 251292-24-7. Formula C17H15NO5. MW 313.3. Synthetic. Cell permeable resorufin derivative used as a fluorogenic indicator for cell viability. Resorufin was selected as the fluorophore because it fluoresces at a longer wavelength and is amenable to modification at the hydroxyl group which is essential for its fluorescence. Incubation with esterase at pH 8.0 results in a 80-90 nm shift of emission max. Ex/Em 500/~593nm in 0.1 M Tris pH 8.0 after cleavage by esterase. This cell viability indicator is 2 times more sensitive than calcein-AM.
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