Edvotek™ Restriction Enzyme Mapping

Description

Includes

instructions, Plasmid DNA for Restriction Digest, Restriction Enzyme Reaction Buffer, UltraPure Water, Restriction Enzyme Dilution Buffer, EcoRI Dryzyme, BamHI Dryzyme, DNA Standard Marker, UltraSpec-Agarose™, Electrophoresis Buffer (50X), 10X Gel Loading Solution, InstaStain™ Ethidium Bromide, FlashBlue™ Stain, Microcentrifuge Tubes with attached caps

Requires

• Requirements:
• Horizontal gel electrophoresis apparatus
• Hotplate, micropipetter, balance
• Waterbath, weigh boats, floating racks
• White light visualization system
• Pipets, pipet pump or bulb
• Glassware, metric rulers, ice
• All necessary safety equipment
• Distilled or deionized water

A prerequisite to cloning and DNA sequencing

In this experiment, a plasmid DNA is cleaved with different combinations of restriction enzymes. By determining the fragment size and using agarose gel electrophoresis, the relative positions of the restriction sites can be mapped.

Specifications

• Product Type: Mapping Kit
• Grade: 9 to Undergraduate
• For Use With (Application): Determining the fragment size and using agarose gel electrophoresis the relative positions of the restriction sites can be mapped
• Includes: Instructions, hind iii and bgl i restriction enzymes, plasmid dna, buffers, enzyme grade water, standard dna fragments, gel loading solution, agarose, electrophoresis buffer, flashblue™ stain
• Quantity: 6 sets of restriction digestions
• Time Range: 1 hour 20 minutes to 1 hour 45 minutes
• Content And Storage: Refrigerated; Store at: -20°C; -4°F
• Class Size: 6 Lab Groups