Promotional price valid on web orders only. Your contract pricing may differ. Interested in signing up for a dedicated account number?
Learn More
Learn More
Edvotek™ Restriction Enzyme Mapping
A prerequisite to cloning and DNA sequencing
Supplier: Edvotek™ 206
Includes: instructions, Plasmid DNA for Restriction Digest, Restriction Enzyme Reaction Buffer, UltraPure Water, Restriction Enzyme Dilution Buffer, EcoRI Dryzyme, BamHI Dryzyme, DNA Standard Marker, UltraSpec-Agarose™, Electrophoresis Buffer (50X), 10X Gel Loading Solution, InstaStain™ Ethidium Bromide, FlashBlue™ Stain, Microcentrifuge Tubes with attached caps
Requires : Requirements:
Horizontal gel electrophoresis apparatus
Hotplate, micropipetter, balance
Waterbath, weigh boats, floating racks
White light visualization system
Pipets, pipet pump or bulb
Glassware, metric rulers, ice
All necessary safety equipment
Distilled or deionized water
Description
In this experiment, a plasmid DNA is cleaved with different combinations of restriction enzymes. By determining the fragment size and using agarose gel electrophoresis, the relative positions of the restriction sites can be mapped.Specifications
| Mapping Kit | |
| Instructions, hind iii and bgl i restriction enzymes, plasmid dna, buffers, enzyme grade water, standard dna fragments, gel loading solution, agarose, electrophoresis buffer, flashblue™ stain | |
| 1 hour 20 minutes to 1 hour 45 minutes |
| Determining the fragment size and using agarose gel electrophoresis the relative positions of the restriction sites can be mapped | |
| 6 sets of restriction digestions | |
| Refrigerated; Store at: -20°C; -4°F |
Provide Content Correction
We continue to work to improve your shopping experience and your feedback regarding this content is very important to us. Please use the form below to provide feedback related to the content on this product.
Product Title
Spot an opportunity for improvement?
Provide Content Correction