Abnova™ RET Split CISH Probe

RET Split CISH Probe is designed for the qualitative detection of human RET gene at 10q11.21 in formalin-fixed, paraffin-embedded specimens by chromogenic in situ hybridization (CISH).

Supplier:  Abnova™ CS0013

Catalog No. 89-335-554

Description

The probe is intended to be used in combination with the CISH Implementation Kit 2, which provides necessary reagents for specimen pretreatment and post-hybridization processing.

Hybridization signals of digoxigenin-labeled polynucleotides appear dark green colored distinct dotshaped (distal to the RET breakpoint region), and dinitrophenyl-labeled polynucleotides appear bright red distinct dot-shaped (proximal to the RET breakpoint region)).
Normal situation: In interphases of normal cells or cells without a translocation involving the RET gene region, two red/green fusion signals appear.
Aberrant situation: One RET gene region affected by a translocation is indicated by one separate green signal and one separate red signal. Isolated green signals are the result of deletions proximal to the RET breakpoint region. Other signal distribution may be observed in some abnormal samples which might result in a different signal pattern than described above, indicating variant rearrangements.
Unexpected signal patterns should be further investigated.

Specifications

• Content And Storage: The product is ready-to-use. No reconstitution, mixing, or dilution is required. Bring probe to room temperature (18 to 25°C) and mix briefly before use.
• For Use With (Application): CISH-Ce