Invitrogen™ Human RET [V804M], GST Tag, His Tag Recombinant Protein

Catalog No.	Quantity
PV6223	10 μg

Catalog No. PV6223 Supplier Invitrogen™ Supplier No. PV6223

Description

Recombinant Protein

For maximum recovery please spin prior to use. Unless noted below, aliquots of the 5 μg, 10 μg and 20 μg sizes of kinase are not recommended as materials can be used in original packaging until exhausted. For larger sizes, the number of freeze/thaws may be reduced by preparing aliquots, aliquots below 20 μL are not recommended. Please never store a kinase diluted. If properly stored at -80C, this product is guaranteed for 6 months from date of purchase. Human RET V804M, C-terminal fragment, amino acids H658-S1114 (as in NCBI/Protein entry NP_066124.1) with a V804M mutation, N-terminal GST-HIS6 fusion protein with a 3C cleavage site, expressed in Sf9 insect cells. Validated in LanthaScreen™ Kinase Binding Assay Protocols available for simple use and optimized performance Multiple pack sizes available for convenient use Related Product:LanthaScreen™ Eu Kinase Binding Assay Protein Form: Recombinant, Fragment, Mutant Assay Entry: Biochemical binding/kinetic.

RET is a member of the cadherin superfamily, encodes one of the receptor tyrosine kinases which are cell-surface molecules that transduce signals for cell growth and differentiation. RET plays a crucial role in neural crest development, can undergo oncogenic activation in vivo and in vitro by cytogenetic rearrangement. Mutations in the RET gene are associated with the disorders multiple endocrine neoplasia, type IIA, multiple endocrine neoplasia, type IIB, Hirschsprung disease, and medullary thyroid carcinoma. Two transcript variants encoding different isoforms have been found for the RET gene. Additional transcript variants have been described for RET but their biological validity has not been confirmed.

Specifications

• Concentration: See Label
• For Use With (Application): Kinase Assay
• Formulation: 50mM HEPES with 20% glycerol, 5mM DTT, 100mM NaCl, 15mM glutathione and no preservative; pH 7.5
• Molecular Weight (g/mol): 80.5 kDa
• Name: Human RET [V804M], GST Tag, His Tag
• pH Range: 7.5
• Purification Method: Purified
• Quantity: 10 μg
• Storage Requirements: -80°C, Avoid Freeze/Thaw Cycles
• Regulatory Status: RUO
• Common Name: RET (V804M Mutant)
• Protein Length: 658-1114
• Product Type: Protein
• Conjugate: Unconjugated
• Species: Human
• Recombinant: Recombinant
• Protein Tag: GST-tag, His-tag
• Sequence: 658-1114
• Content And Storage: -80°C, Avoid Freeze/Thaw Cycles
• Assay Entry: Biochemical binding/kinetic
• Kinase Group: Receptor Tyrosine Kinase
• Target Entry: RET V804M
• Expression System: Insect cells
• Protein Family: Kinases & Inhibitors
• Protein Form: Recombinant, Fragment, Mutant
• Form: Liquid