Invitrogen™ Reverse T3 Competitive ELISA Kit

Catalog No.	Quantity
EEL137	96 Tests

Catalog No. EEL137 Supplier Invitrogen™ Supplier No. EEL137

Description

ELISA

Reverse triiodothyronine (rT3) is a biologically inactive form of the thyroid hormone triiodothyronine (T3). rT3 is produced by the enzymatic deiodination of T4, primarily in peripheral tissues, and is mainly found in the liver and kidneys. Its primary function is to regulate the metabolism of T3 by blocking its action. The structure of rT3 is similar to that of T3, with the exception of the position of one iodine atom. This structural difference causes rT3 to be biologically inactive and unable to bind to thyroid hormone receptors. Despite its lack of direct physiological activity, rT3 levels can be used as a marker of thyroid hormone status and metabolic function.

Specifications

• Assay Range: 93.75 to 6000 pg/mL
• Assay Sensitivity: 56.25 pg/mL
• Conjugate: HRP
• Product Type: ELISA
• Sample Type: Plasma, Serum
• For Use With (Equipment): Colorimetric Microplate Reader
• Interassay CV: <10%
• Intraassay CV: <10
• Kit Contents: Pre-coated 96 well plate, Standard, Biotinylated Detection Antibody, HRP Conjugate, Standard & Sample Diluent, Biotinylated Detection Antibody Diluent, HRP Conjugate Diluent, Wash Buffer, TMB Substrate, Stop Solution, Plate Sealer
• Label or Dye: HRP
• Quantity: 96 Tests
• Regulatory Status: RUO
• Sample Volume: Plasma 50 μL, Serum 50 μL
• Storage Requirements: Refer to product documentation for component specific storage temperature.
• Target: Reverse T3
• Test Time: 1 hrs 20 mins
• Total Assay Time: 3 hrs 30 mins