Invitrogen™ Reverse T3 Competitive ELISA Kit

Catalog No.	Quantity
EEL137	96 Tests

Catalog No. EEL137 Supplier Invitrogen™ Supplier No. EEL137

Description

ELISA

The multispecies Reverse Triiodothyronine (rT3) ELISA quantitates rT3 in serum, plasma, and other biological fluids Principle of the method: The Competitive multispecies ELISA research-use-only kit is designed to quantitatively measure Reverse Triiodothyronine (rT3) independent of species. A rT3 standard is provided to generate a standard curve for the assay and all samples are read off a user-generated standard curve. Standards or diluted samples are pipetted into a coated microtiter plate and Biotinylated Detection Ab specific to rT3 added. Excess conjugate and unbound sample or standard are washed from the plate, and Avidin conjugated to Horseradish Peroxidase (HRP) is added to each well and incubated. Then a TMB substrate solution is added to each well. The enzyme-substrate reaction is terminated by the addition of stop solution and the color change is measured on a microplate reader. The concentration of rT3 in the samples is then determined by comparing the OD of the samples to the standard curve. Rigorous validation: Each manufactured lot of this ELISA kit is quality tested for criteria such as sensitivity, specificity, precision, and lot-to-lot consistency. See manual for more information on validation.

Reverse triiodothyronine (rT3) is a biologically inactive form of the thyroid hormone triiodothyronine (T3). rT3 is produced by the enzymatic deiodination of T4, primarily in peripheral tissues, and is mainly found in the liver and kidneys. Its primary function is to regulate the metabolism of T3 by blocking its action. The structure of rT3 is similar to that of T3, with the exception of the position of one iodine atom. This structural difference causes rT3 to be biologically inactive and unable to bind to thyroid hormone receptors. Despite its lack of direct physiological activity, rT3 levels can be used as a marker of thyroid hormone status and metabolic function.

Specifications

• Assay Range: 93.75-6000 pg/mL
• Assay Sensitivity: 56.25 pg/mL
• Conjugate: HRP
• Immunoassay Kit Format: Sandwich ELISA Kit
• Product Type: ELISA
• Protein Family: Thyroid hormone
• Sample Type: Plasma, Serum
• Target Species: All Species
• For Use With (Equipment): Absorbance Microplate Reader
• Gene Alias: Reverse triiodothyronineReverse triiodothyronine;
• Interassay CV: <10%
• Intraassay CV: <10%
• Kit Contents: Pre-coated 96 well plate, Standard, Biotinylated Detection Antibody, HRP Conjugate, Standard & Sample Diluent, Biotinylated Detection Antibody Diluent, HRP Conjugate Diluent, Wash Buffer, TMB Substrate, Stop Solution, Plate Sealer, Standard, Biotinylated Detection Antibody, HRP Conjugate, Standard & Sample Diluent, Biotinylated Detection Antibody Diluent, HRP Conjugate Diluent, Wash Buffer, TMB Substrate, Stop Solution, Plate Sealer
• Label or Dye: Biotin
• Quantity: 96 Tests
• Regulatory Status: RUO
• Sample Volume: Plasma 50 μL, Serum 50 μL
• Shipping Condition: Wet or Dry Ice
• Storage Requirements: Refer to product documentation for component specific storage temperature.
• Target: Reverse T3
• Test Time: 1 hr 20 min
• Total Assay Time: 2 hr 30 min