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Ambion™ RiboMinus™ Eukaryote Kit for RNA-Seq

Catalog No. A1083708
Encompass_Preferred
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Quantity:
8 Preps (10 μg⁄prep)

The RiboMinus™ Eukaryote Kit for RNA-Seq provides truly complete transcriptome isolation for next generation sequencing analysis.

The RiboMinus™ Eukaryote Kit for RNA-Seq provides truly complete transcriptome isolation for next generation sequencing analysis by offering a method for selective depletion of up to 99% of the 5S, 5.8S, 18S and 28S ribosomal RNA from total RNA versus poly-A or cap binding methods, which can introduce bias and select for only part of the transcriptome.

The RiboMinus™ Eukaryote Kit for RNA-Seq Offers:
• A simple, yet selective depletion procedure that uses a sequence-specific probe to capture rRNA
• Rapid and efficient removal of rRNA-probe complex using magnetic bead technology

Three Steps to an rRNA-free Preparation Suitable for Transcriptome Analysis
The removal of large ribosomal RNA using the RiboMinus™ kit is a reliable and robust three-step procedure. Hybridize the RNA sample to the RiboMinus™ Probe in a water bath or heat block, then remove probe-rRNA complexes using the RiboMinus™ magnetic beads included in the kit. Concentrate the depleted RNA preparation by ethanol precipitation, or by using the RiboMinus™ Concentration Module (Cat. No. K155005).

TRUSTED_SUSTAINABILITY

Specifications

Content And Storage

Each kit contains:
• 6 mL RiboMinus™ Magnetic Beads
• 80 μL RiboMinus™ Eukaryote Probe (15 pmol/μL)
• 10 mL Hybridization Buffer
• 12 mL DEPC-treated (RNase-free) water

Store at 4°C.

Isolation Technology Magnetic Bead
Sample Type Total RNA
Final Product Type Transcriptome RNA
High-throughput Compatibility High-throughput Compatible
For Use With (Application) Microarray Analysis, Next-Generation Sequencing, Real-Time Quantitative PCR (qPCR), Reverse Transcriptase PCR (RT-PCR), Sequencing, Northern Blotting, cDNA Library Construction
No. of Reactions 8 Preps
Product Line RiboMinus™
Quantity 8 Preps (10 μg⁄prep)
Sequencing Type Transcriptome Sequencing
Shipping Condition Wet Ice
Starting Material Amount 2-10 μg Total RNA
Target Transcriptome RNA
System Type RiboMinus
Yield 1 μg
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For Research Use Only. Not for use in diagnostic procedures.

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