Ambion™ RiboMinus™ Eukaryote Kit for RNA-Seq

Description

The RiboMinus™ Eukaryote Kit for RNA-Seq provides truly complete transcriptome isolation for next generation sequencing analysis.

The RiboMinus™ Eukaryote Kit for RNA-Seq provides truly complete transcriptome isolation for next generation sequencing analysis by offering a method for selective depletion of up to 99% of the 5S, 5.8S, 18S and 28S ribosomal RNA from total RNA versus poly-A or cap binding methods, which can introduce bias and select for only part of the transcriptome.

The RiboMinus™ Eukaryote Kit for RNA-Seq Offers:
• A simple, yet selective depletion procedure that uses a sequence-specific probe to capture rRNA
• Rapid and efficient removal of rRNA-probe complex using magnetic bead technology

Three Steps to an rRNA-free Preparation Suitable for Transcriptome Analysis
The removal of large ribosomal RNA using the RiboMinus™ kit is a reliable and robust three-step procedure. Hybridize the RNA sample to the RiboMinus™ Probe in a water bath or heat block, then remove probe-rRNA complexes using the RiboMinus™ magnetic beads included in the kit. Concentrate the depleted RNA preparation by ethanol precipitation, or by using the RiboMinus™ Concentration Module (Cat. No. K155005).

Specifications

• Content And Storage:

  Each kit contains:
  • 6 mL RiboMinus™ Magnetic Beads
  • 80 μL RiboMinus™ Eukaryote Probe (15 pmol/μL)
  • 10 mL Hybridization Buffer
  • 12 mL DEPC-treated (RNase-free) water
  
  Store at 4°C.

• Isolation Technology: Magnetic Bead
• Sample Type: Total RNA
• Final Product Type: Transcriptome RNA
• High-throughput Compatibility: High-throughput Compatible
• For Use With (Application): Microarray Analysis, Next-Generation Sequencing, Real-Time Quantitative PCR (qPCR), Reverse Transcriptase PCR (RT-PCR), Sequencing, Northern Blotting, cDNA Library Construction
• No. of Reactions: 8 Preps
• Product Line: RiboMinus™
• Quantity: 8 Preps (10 μg⁄prep)
• Sequencing Type: Transcriptome Sequencing
• Shipping Condition: Wet Ice
• Starting Material Amount: 2-10 μg Total RNA
• Target: Transcriptome RNA
• System Type: RiboMinus
• Yield: 1 μg

For Research Use Only. Not for use in diagnostic procedures.