Invitrogen™ RiboMinus™ Eukaryote Kit v2

Description

The RiboMinus™ Eukaryote Kit v2 improves upon the previous system (RiboMinus™ Eukaryote Kit for RNA-Seq) by removing significantly more rRNA (both cytoplasmic and mitochondrial) from the total RNA.

Features of the kit include:
• Seamless integration with RiboMinus™Concentration Module (available separately) to purify RNA after ribosomal RNA depletion
• 1.5-hour workflow
• Input range of 1-5 μg

The RiboMinus™ Eukaryote Kit v2 improves upon the previous system (RiboMinus™ Eukaryote Kit for RNA-Seq) by removing significantly more rRNA (both cytoplasmic and mitochondrial) from the total RNA. When used with our library construction kits for sequencing, the RiboMinus™ Eukaryote Kit v2 provides maximum mapping to reference database with minimum bias, maintaining relative gene expression levels of even smaller RNA species. The resulting total RNA after depletion contains minimum contaminating rRNA with reduced variability.

The RiboMinus Eukaryote Kit v2 is designed for the removal of human, mouse, and rat rRNA. The RiboMinus™ Concentration Module (available separately) is used for RNA purification after rRNA depletion. For magnetic bead-based purification, please see the RiboMinus™ Eukaryote System v2. The RiboMinus Eukaryote Kit v2 has an RNA input range of 1-5 μg. For lower inputs (down to 100 ng), the Low Input RiboMinus™ Eukaryote System v2 is recommended.

The RiboMinus™ Eukaryote Kit v2 contains sufficient reagents to perform 12 ribosomal RNA depletions.

Specifications

• Content And Storage:

  • RiboMinus™ Eukaryote Probe Mix, 2-20°C
  • RiboMinus™ Magnetic Beads, 4°C
  • 2 x Hybridization Buffer, room temperature

• Isolation Technology: Magnetic Bead
• Species: Human, Mouse, Rat
• Final Product Type: Transcriptome RNA
• For Use With (Equipment): Ion PGM™ System, Ion Proton™ System, Illumina™ Genome Analyzer II (GA II)
• Product Line: RiboMinus™
• Quantity: 12 reactions
• Sequencing Type: Transcriptome Sequencing
• Starting Material Amount: 1 - 5 μg total RNA
• Workflow Step: Sample Enrichment

For Research Use Only. Not for use in diagnostic procedures.