Ribonuclease A bovine pancreas is an endoribonuclease targeting RNA It is designed to catalyze the cleavage of phosphodiester bonds adjacent to pyrimidine nucleotides within RNA thereby facilitating the degradation of RNA strands Ribonuclease A bovine pancreas exerts its biological activity primarily through the specific cleavage of the 3 -phosphate bond of pyrimidine residues in RNA generating pyrimidine-terminated oligonucleotides and 3 -phosphates Its activity varies with ionic strength digesting single-stranded RNA double-stranded RNA and RNA/DNA hybrids at low salt concentrations ( 100 mM NaCl) and preferentially cleaving single-stranded RNA at higher salt concentrations ( 0 3 M NaCl) Based on these biochemical properties Ribonuclease A bovine pancreas holds research potential in removal of RNA contamination from plasmid and genomic DNA preparations RNase protection assays and RNA sequence analysis