Thermo Scientific™ RIPA Extraction Bundle

Catalog No. PIB90004

Supplier: Thermo Scientific™ B90004

Description

Includes

RIPA Lysis and Extraction Buffer (100 mL), Halt Protease Inhibitor Cocktail (100X) (1 mL), Pierce Rapid Gold BCA Protein Assay Kit (250 mL)

RIPA Extraction Bundle effectively lyses and extracts proteins from cultured mammalian cells and provides high-precision total protein concentration determination. The bundle includes RIPA Lysis and Extraction Buffer, Halt Protease Inhibitor Cocktail and Pierce Rapid Gold BCA Protein Assay kit

• Thermo Scientific RIPA Lysis and Extraction Buffer is a high-quality, ready-to-use RIPA buffer that effectively lyses and extracts protein from cultured mammalian cells, including plated cells and pelleted suspension cells. The popular reagent enables the extraction of membrane, nuclear and cytoplasmic proteins and is compatible with many applications, including reporter assays, immunoassays and protein purification
• Thermo Scientific Halt Protease Inhibitor Cocktail with RIPA buffer, added before use, protects proteins from proteolytic degradation through inhibition of serine proteases, cysteine proteases, aspartic acid proteases, and aminopeptidases that are typically present in cell lysate samples
• The Thermo Scientific Pierce Rapid Gold BCA Protein Assay Kit is a two-component, high-precision, detergent-compatible assay optimized to measure (at 480nm) total protein concentration compared to a protein standard curve of known concentrations. The Pierce Rapid Gold BCA Protein Assay uses the same copper-chelating technology as the well-known traditional Pierce BCA Protein Assay and provides comparable accuracy but with a 5 minute, room temperature incubation. This improvement eliminates the need to wait or expose the samples to elevated temperatures for a fast time to results

Specifications

• Quantity: 1 Bundle
• Includes: RIPA Lysis and Extraction Buffer (100 mL), Halt Protease Inhibitor Cocktail (100X) (1 mL), Pierce Rapid Gold BCA Protein Assay Kit (250 mL)
• Content And Storage: See instructions for individual components