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ABclonal Technology T4 RNA Ligase 2, truncated KQ
Supplier: ABclonal Technology RK2050410000U
T4 RNA Ligase 2, truncated KQ (T4 Rnl2tr R55K, K227Q), specifically catalyzes the ligation of a 5-adenylated DNA or RNA to the 3 OH end of an RNA molecule. This enzyme-mediated ligation does not require ATP but necessitates a pre-adenylated adapter. T4 RNA Ligase 2, truncated KQ, a double-point mutant of T4 RNA Ligase 2 with R55K and K227Q substitutions, significantly reduces the non-specific ligation of RNA (such as concatenation or self-circularization) compared to T4 RNA Ligase 2, truncated, while maintaining enzyme activity similar to that of T4 RNA Ligase 2, truncated.
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