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ABclonal Technology 5'App-T4 DNA Ligase

Supplier: ABclonal Technology RK2051050000U
5App-T4 DNA Ligase is a mutant of T4 DNA Ligase without adenylation function, which can specifically ligates the pre-adenylated 5? end of DNA or RNA to the 3? end of DNA. This enzyme does not require ATP as a cofactor for ligation, but requires a pre-adenylated substrate. This enzyme reduces background ligation (chimera formation) during NGS library construction, because it can only use 5 preadenylation adapter to the DNA fragments.
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