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ABclonal Technology T4 Polynucleotide Kinase
Supplier: ABclonal Technology RK20524250U
T4 Polynucleotide Kinase (T4 PNK) catalyzes the transfer and exchange of Pi from the γ position of ATP to the 5 -hydroxyl terminus of polynucleotides (double-and single-stranded DNA and RNA) and nucleoside 3 -monophosphates. T4 Polynucleotide Kinase also catalyzes the removal of 3 -phosphoryl groups from 3 -phosphoryl polynucleotides, deoxynucleoside 3 -monophosphates and deoxynucleoside 3 -diphosphates. T4 PNK is applicable to end-labeling DNA or RNA for probes and DNA sequencing, addition of 5 -phosphates to oligonucleotides to allow subsequent ligation and removal of 3 -phosphoryl groups.
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