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ABclonal Technology T4 DNA Polymerase (5,000 U/mL)
Supplier: ABclonal Technology RK205391000U
T4 DNA Polymerase catalyzes the synthesis of DNA in the 5to3 direction and requires the presence of template and primer. This enzyme has a 3to5 exonuclease activity which is much more active than that found in DNA Polymerase I (E. coli). Unlike E. coli DNA Polymerase I, T4 DNA Polymerase does not have a 5to3 exonuclease function. It is applicable to 3 overhang removal to form blunt ends, 5 overhang fill-in to form blunt ends, single strand deletion subcloning, second strand synthesis in site-directed mutagenesis and probe labeling using replacement synthesis.
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