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ABclonal Technology Heat-T4 DNA Ligase
Supplier: ABclonal Technology RK2150720000U
Heat-T4 DNA Ligase is a thermostable mutant of T4 DNA Ligase that catalyzes the formation of a phosphodiester bond between juxtaposed 5? phosphate and 3? hydroxyl termini in duplex DNA or RNA. It is designed to function at higher temperatures than wild-type T4 DNA ligase. The catalytic reaction requires ATP as a cofactor. Heat-T4 DNA Ligase will join blunt end and cohesive end termini at temperatures as high as 45?C , and can also repair single-stranded nicks in duplex DNA, RNA or DNA/RNA hybrids. At the same time, T4 DNA ligase closes the gaps in these DNA substrates.
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