ABclonal Technology AFTSpin Plasmid Mini Kit

Supplier:  ABclonal Technology RK3010150RXN

The kit is capable of lysing cells using a modified SDS-alkaline lysis method. The optimized lysate allows the silica-based membrane in the centrifugal adsorption column to selectively bind the plasmid DNA in the solution under the condition of high salt and low pH value and then removes impurities and other bacterial components by deproteinizing solution PR and washing solution WB. Finally the low-salt, high-pH eluent EB elutes the pure plasmid DNA from the silica-based membrane.
In general, a high-copy plasmid is inoculated from a single colony in 1.5-4.5 mL of LB medium supplemented with appropriate antibiotics. After overnight culture for 12-16 hours, up to 20 ug of pure plasmid can be extracted.

Catalog No. 50-001-38045