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ABclonal Technology AFTSpin EndoFree Plasmid Maxi Kit
Supplier: ABclonal Technology RK3010310RXN
This kit is capable of lysing cells using a modified SDS-alkaline lysis method. The crude extract is selectively bound with a unique endotoxin scavenger and centrifuged to remove the endotoxin. The silica-based membrane in the centrifugal adsorption column can bind the plasmid DNA in the solution selectively under the condition of high salt and low pH value, and then impurities and other bacterial components can be removed by the deproteinizing solution PR and washing solution WB. Finally the low-salt, high-pH eluent EB elutes pure plasmid DNA from the silica-based membrane.
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