New England Biolabs, Inc. Monarch RNase A - 1 ml (2x0.5ml)

Supplier:  New England Biolabs, Inc. T3018L

Monarch RNase A, a component of the Monarch Genomic DNA Purification Kit, can be used to purify genomic DNA from a variety of sample types. It is used at 56°C in the kit protocol but is active at any temperature 20-65°C. Treatment with RNase A is an optional step in the Monarch Genomic DNA Purification Kit protocol for removal of any residual RNA. Co-purification of RNA during gDNA extractions is a common problem that leads to the overestimation of DNA yield. The low alcohol binding conditions employed in the kit optimizes binding of gDNA alone, so RNA co-purification is extremely low.

• Monarch RNase A degrades single-stranded RNA at C and U residues
• Purified from cow pancreas and is free of contaminating protease or DNase activity
• Contains no residual host DNA
• It is supplied as a 20 mg/ml solution in 50% glycerol, 50 mM Tris-Cl pH 7.4
• Monarch kit components are also available separately for added convenience

Catalog No. NC1685115