New England Biolabs, Inc. Monarch RNase A – 1 ml (2x0.5ml)

Supplier:  New England Biolabs, Inc. T3018L

Monarch RNase A is a component of the Monarch Genomic DNA Purification Kit, which can be used to purify genomic DNA from a variety of sample types. Monarch RNase A degrades single-stranded RNA at C and U residues. This formulation is purified from cow pancreas, is free of contaminating protease or DNase activity, and contains no residual host DNA. It is used at 56C in the Monarch Genomic DNA Purification Kit protocol but is active at any temperature 20-65C. It is supplied as a 20 mg/ml solution in 50% glycerol, 50 mM Tris-Cl pH 7.4. Treatment with RNase A is an optional step in the Monarch Genomic DNA Purification Kit protocol for removal of any residual RNA. Co-purification of RNA during gDNA extractions is a common problem that leads to the overestimation of DNA yield. The low alcohol binding conditions employed in the kit optimizes binding of gDNA alone, so RNA co-purification is extremely low.

Catalog No. NC1685115