Sigma Aldrich Fine Chemicals Biosciences RNAzolR RT for processing

Supplier:  Sigma Aldrich Fine Chemicals Biosciences R4533200ML

RNAzol RT is a quick and convenient reagent for use in thesingle-step isolation of total andsmall RNA from biological samples of human animal plant yeast bacterial and viral origin. A convenient single-step liquid phase separation results in the isolation of RNA from DNA protein polysaccharides and other molecules. RNAzol(R) RT can be used to isolate separate fractions of mRNA and micro RNA or to isolate total RNA containing all classes of RNA in a single fraction. This product a mixture of guanidine thiocyanate and phenol in a monophase SOLUTIONon effectively dissolves DNA RNA and protein on homogenization or lysis of tissue sample. Addition of water to the mixture allows for the precipitation of DNA proteins polysaccharides and other molecules which can then be removed by centrifugation. RNA can then be isolated by alcohol precipitation washing and solubilization. Chloroform-induced phase separation is not necessary. One mL of RNAzol(R) RT is sufficient to isolate RN

Catalog No. 50-290-5422