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STEMCELL Technologies RoboSep™ Human Monocyte Enrichment Kit without CD16 Depletion with Filter Tips, For processing: 1 x 10^9 cells
Supplier: STEMCELL Technologies 19058RF
The EasySep™ Human Monocyte Enrichment Kit without CD16 Depletion is designed to isolate monocytes and CD14+CD16+ monocytes from fresh or previously frozen peripheral blood mononuclear cells by negative selection. The CD14+CD16+ subset of monocytes (~10% in blood of healthy individuals) has characteristics of tissue macrophages and expands greatly in acute and chronic inflammatory disease. Unwanted cells are targeted for removal with Tetrameric Antibody Complexes recognizing non-monocyte cells and magnetic particles. The cocktail also contains an antibody to human Fc receptor to minimize nonspecific binding. The labeled cells are separated using an EasySep™ magnet without the use of columns. Desired cells are poured off into a new tube. For applications in which removal of all CD16+ cells is desired, we recommend the EasySep™ Human Monocyte Enrichment Kit (/products/easysep-human-monocyte-isolation-kit.html) (Catalog #19359), which contains anti-CD16.
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