Abnova™ ROS1 Split CISH Probe

Catalog No.	For Use With (Application)
89-335-555	CISH-P,CISH-Ce

Catalog No. 89-335-555 Supplier Abnova™ Supplier No. CS0014

Description

ROS1 Split CISH Probe is designed for the qualitative detection of human ROS1 gene at 6q22.1 in formalin-fixed, paraffin-embedded specimens by chromogenic in situ hybridization (CISH).

The probe is intended to be used in combination with the CISH Implementation Kit 2, which provides necessary reagents for specimen pretreatment and post-hybridization processing.

Hybridization signals of digoxigenin-labeled polynucleotides appear dark green colored distinct dotshaped (proximal to the ROS1 breakpoint region), and dinitrophenyl-labeled polynucleotides appear bright red distinct dot-shaped (distal to the ROS1 breakpoint region).
Normal situation: In interphases of normal cells or cells without a translocation involving the ROS1 gene region, two red/green fusion signals appear.
Aberrant situation: One ROS1 gene region affected by a translocation is indicated by one separate green signal and one separate red signal. Isolated green signals are the result of deletions distal to the ROS1 breakpoint region or are due to unbalanced translocations affecting this chromosomal region. Other signal distribution may be observed in some abnormal samples which might result in a different signal pattern than described above, indicating variant rearrangements.
Unexpected signal patterns should be further investigated.

Specifications

• For Use With (Application): CISH-P,CISH-Ce
• Content And Storage: The product is ready-to-use. No reconstitution, mixing, or dilution is required. Bring probe to room temperature (18 to 25°C) and mix briefly before use.