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Meridian Life Science RT-qPCR Extraction Control, 1ML
Supplier: Meridian Life Science MDX0291
Molecular IVD companies continue to experience tighter and tighter regulations in regard to incorporating appropriate positive and negative controls into their assays. Meridian’s RT-qPCR Extraction Control Orange uses artificial micelles that contain quantitative PCR positive control RNA sequence (with no known homology to any organism, so that it does not interfere with the detection of the sample target RNA). The cells are added to the RT-qPCR Extraction Control is spiked in the with lysis buffer into the sample prior to RNA extraction for downstream amplification by RT-qPCR. The detection of RT-qPCR Extraction Control acts as a cDNA synthesis and PCR positive control and confirms the success of the extraction and amplification steps, avoiding the misinterpretation of a false negative result.
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