HyperScript RT SuperMix for qPCR is a reverse transcription reaction premix based on HyperScript Reverse Transcriptase a novel enzyme engineered from M-MLV (RNase H-) Reverse Transcriptase featuring significantly reduced RNase H activity and greatly enhanced thermo-stability Due to its improved heat tolerance HyperScript Reverse Transcriptase is especially suitable for reverse transcription of RNA templates with complex secondary structures HyperScript RT SuperMix for qPCR (with gDNA wiper) is specifically designed for two-step qRT-PCR assays The 5 SuperMix contains all necessary components for reverse transcription Simply adding template RNA and RNase-Free ddH2O allows rapid reaction initiation The included 4 gDNA wiper efficiently eliminates potential genomic DNA contamination from samples rapidly and thoroughly prior to reverse transcription The kit is particularly suitable for reverse transcription reactions from RNA templates of low concentration