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RY743 Mouse Anti-Mouse IgM[b], BD OptiBuild™

Catalog No. BDB773062 Shop All BD Biosciences Products
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BDB773062 50 μg
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Catalog No. BDB773062 Supplier BD Biosciences Supplier No. 773062
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Description

Mouse Monoclonal Secondary Antibody

Monoclonal Antibody Specifically detects in Mouse Samples. It is validated for Flow Cytometry.

The AF6-78 antibody reacts specifically with mouse IgM of Igh-C[b] and related haplotypes (e.g., C57BL/6, C57BL/10, SJL, AKR, NZB). It does not react with IgM of Igh-C[a] or related haplotypes (e.g., BALB/c, C58, CBA, C3H/Bi, C3H/He, DBA/1, DBA/2, PL, RIII). Cross-reaction with IgM of Igh-C[e] haplotype (e.g., A/J) has been observed. AF6-78 antibody does not react with free μ heavy chain in vitro or in the cytoplasm of pre-B lymphocytes, which lack Ig light chain. It has not been shown to stimulate B-cell proliferation. The BD Horizon RealYellowTM 743 (RY743) Dye is part of the BD™ family of yellow-green dyes. It is a tandem fluorochrome with an excitation maximum (Ex Max) at 557 nm and an emission maximum (Em Max) at 743 nm as measured using an antibody-dye conjugate. It is optimized for detection on spectral cytometers and designed to be excited by the Yellow-Green laser (561 nm) with minimal excitation by the Blue laser (488 nm).
TRUSTED_SUSTAINABILITY

Specifications

Applications Flow Cytometry
Classification Monoclonal
Clone AF6-78
Concentration 0.2 mg/mL
Conjugate RY743
Formulation Aqueous buffered solution containing ≤0.09% sodium azide.
Gene Alias Igh-6b
Host Species Mouse
Immunogen C57BL/10 mouse splenocytes
Quantity 50 μg
Regulatory Status RUO
Primary or Secondary Secondary
Gene ID (Entrez) 16019
Target Species Mouse
Content And Storage Store undiluted at 4°C and protected from prolonged exposure to light. Do not freeze. The monoclonal antibody was purified from tissue culture supernatant or ascites by affinity chromatography. The antibody was conjugated to the dye under optimum conditions that minimize unconjugated dye and antibody.
Product Type Antibody
Form Liquid
Isotype IgG1 κ
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