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Raybiotech Inc RAYBIOTECH INC S-Acylation Detection Kit, Biotin Switch, 40 Sample Kit
Supplier: Raybiotech Inc MAACYLW
The S-Acylation Detection Kit employs a modified biotin-switch method to enable direct visualization of S-acylation, including S-palmitoylation, of proteins via western blot analysis. This kit is capable of biotin-labeling 40 samples, each containing 100-200 μg of total protein.
- Utilizes modified biotin-switch method for S-acylation visualization.
- Detects S-palmitoylation of proteins via western blot analysis.
- Biotin-labels 40 samples, each with 100 to 200 μg of protein.
- Employs acyl-biotinyl exchange (ABE) method.
- Blocks unmodified free cysteines, then selectively labels acylated cysteines.
- Biotinylation detected by western blot or immunoassay.
- Avidin resin enriches biotin-labeled acylated proteins/peptides.
- Facilitates S-acylation/palmitoylation site mapping via mass spectrometry.
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