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Apexbio Technology LLC Single Firefly Luciferase Reporter Gene Assay Kit II 100tests
Supplier: Apexbio Technology LLC K2246100
The luciferase-luciferin bioluminescent system provides a highly sensitive and efficient tool for detecting gene expression In the presence of ATP oxygen and Mg2 firefly luciferase catalyzes the oxidation reaction of luciferin into oxyluciferin accompanied by the emission of bioluminescence at about 560 nm following detection by a luminometer or liquid scintillation counter The Single Firefly Luciferase Reporter Gene Assay Kit allows direct quantification of intracellular firefly luciferase activity via chemiluminescence without the need for washing or harvesting cells This assay is a one-step detection kit providing exceptional sensitivity high signal stability and ease of use This product is an alternative lyophilized powder version of Kit (K2236) with the same performance offering the benefit of long-term stable storage at -20 C Before use the lyophilized substrate powder needs to be fully dissolved with the buffer provided in the kit to prepare working solution
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