Recombinant Protein;100UG;SARS-CoV-2 S protein RBD (K417N,E484K,N501Y), Fc Tag (SPD-C5256) is expressed from human 293 cells (HEK293). It contains AA Arg 319 - Lys 537 (Accession # QHD43416.1 (K417N, E484K, N501Y)). The K417N, E484K, N501Y mutations were identified in the SARS-CoV-2 variant (known as B.1.351 or 20C/501Y.V2) which emerged in South Africa.;This protein carries a human IgG1 Fc tag at the C-terminus. The protein has a calculated MW of 51.1 kDa. The protein migrates as 55-60 kDa under reducing (R) condition (SDS-PAGE) due to glycosylation.;Immobilized SARS-CoV-2 S protein RBD (K417N, E484K, N501Y), Fc Tag (Cat. No. SPD-C5256) at 2 μg/mL (100 μL/well) can bind Human ACE2, His Tag (Cat. No. AC2-H52H8) with a linear range of 39-156 ng/mL (QC tested).;Immobilized Human ACE2, His Tag (Cat. No. AC2-H52H8) at 2 μg/mL (100 μL/well) can bind SARS-CoV-2 S protein RBD (K417N, E484K, N501Y), Fc Tag (Cat. No. SPD-C5256) with a linear range of 0.1-0.8 ng/mL (Routinely tested).