Recombinant Protein;1MG;SARS-CoV-2 S protein RBD (K417T, E484K, N501Y), His Tag (SPD-C52Hr) is expressed from human 293 cells (HEK293). It contains AA Arg 319 - Lys 537 (Accession # QHD43416.1 (K417T, E484K, N501Y)). K417T/ E484K/ N501Y mutations were identified in the SARS-CoV-2 variant (known as variant P.1) which emerged in Brazil.;This protein carries a polyhistidine tag at the C-terminus. The protein has a calculated MW of 26.5 kDa. The protein migrates as 33-35 kDa under reducing (R) condition (SDS-PAGE) due to glycosylation.;Immobilized SARS-CoV-2 S protein RBD (K417T, E484K, N501Y), His Tag (Cat. No. SPD-C52Hr) at 1 μg/mL (100 μL/well) can bind Human ACE2, Fc Tag (Cat. No. AC2-H5257) with a linear range of 0.8-3 ng/mL (QC tested).;Immobilized Human ACE2, Fc Tag (Cat. No. AC2-H5257) at 1 μg/mL (100 μL/well) can bind SARS-CoV-2 S protein RBD (K417T, E484K, N501Y), His Tag (Cat. No. SPD-C52Hr) with a linear range of 2-39 ng/mL (Routinely tested).