Invitrogen™ SARS-CoV-2 Spike Protein (RBD) (K417T) Fc Chimera Recombinant Protein

Catalog No.	Quantity
PIRP88310	100 μg

Catalog No. PIRP88310 Supplier Invitrogen™ Supplier No. RP88310

Description

Recombinant Protein

It is recommended that the protein be aliquoted for optimal storage. A DNA sequence encoding the SARS-CoV-2 (2019-nCoV) Spike RBD (YP_009724390.1, with mutation K417T) (Arg319-Phe541) was expressed with the Fc region of rabbit IgG1 at the C-terminus. It is recommended that sterile water (400 μL) be added to the vial to prepare a stock solution of 0.25 mg/mL. Concentration is measured by UV.

SARS-CoV-2 (Severe Acute Respiratory Syndrome Coronavirus 2) also known as 2019-nCoV (2019 Novel Coronavirus) is a virus that causes illnesses ranging from the common cold symptoms to severe effects including death and respiratory distress. The CoV Spike (S) protein plays the most important roles in viral attachment, fusion and entry, and serves as a target for development of antibodies, entry inhibitors and vaccines. Of note is the Spike protein receptor-binding domain (RBD, S-RBD) in SARS-CoV-2 Spike protein which binds strongly to human and bat angiotensin-converting enzyme 2 (ACE2) receptors. SARS-CoV-2 RBD exhibits significantly higher binding affinity to ACE2 receptor than SARS-CoV RBD in comparison. S1 contains a receptor-binding domain (RBD) that can specifically bind to angiotensin-converting enzyme 2 (ACE2), the receptor on target cells.

Specifications

• For Use With (Application): Control, Neutralization
• Format: Lyophilized
• Formulation: PBS with 0.01% mannitol, 5-8% trehalose, 0.01% Tween 80 and no preservative; pH 7.4
• Molecular Weight (g/mol): 56.5 kDa
• Name: SARS-CoV-2 Spike Protein (RBD) (K417T) Fc Chimera
• pH Range: 7.4
• Purification Method: Purified
• Quantity: 100 μg
• Storage Requirements: -20°C, Avoid Freeze/Thaw Cycles
• Regulatory Status: RUO
• Endotoxin Concentration: <1.0 EU/μg
• Gene Alias: Coronavirus; COVID; Covid 19; COVID19; COVID-19; SARS; SARS Coronavirus; SARS CoV; SARS CoV2; SARS CoV-2; SARS Spike Protein; SARS-CoV2; SARS-CoV-2
• Common Name: SARS-CoV-2 Spike Protein RBD
• Product Type: Protein
• Conjugate: Unconjugated
• Recombinant: Recombinant
• Protein Tag: Fc-tag
• Sequence: SARS-CoV-2 spike RBD (Accession # YP_009724390.1), amino acids Arg319-Phe541 with a K417T substitution and a C-terminal rabbit IgG1 Fc region
• Content And Storage: -20°C, Avoid Freeze/Thaw Cycles
• Expression System: HEK293 cells
• Form: Lyophilized
• Purity or Quality Grade: >95% as determined by SDS-PAGE.