Invitrogen™ SARS-CoV-2 Spike Protein (RBD) Recombinant Protein

Catalog No.	Quantity
PIRP88317	100 μg

Catalog No. PIRP88317 Supplier Invitrogen™ Supplier No. RP88317

Description

Recombinant Protein

It is recommended that the protein be aliquoted for optimal storage. It is recommended that sterile water (400 μL) be added to the vial to prepare a stock solution of 0.25 mg/mL. Concentration is measured by UV.

SARS-CoV-2 (Severe Acute Respiratory Syndrome Coronavirus 2) also known as 2019-nCoV (2019 Novel Coronavirus) is a virus that causes illnesses ranging from the common cold symptoms to severe effects including death and respiratory distress. The CoV Spike (S) protein plays the most important roles in viral attachment, fusion and entry, and serves as a target for development of antibodies, entry inhibitors and vaccines. Of note is the Spike protein receptor-binding domain (RBD, S-RBD) in SARS-CoV-2 Spike protein which binds strongly to human and bat angiotensin-converting enzyme 2 (ACE2) receptors. SARS-CoV-2 RBD exhibits significantly higher binding affinity to ACE2 receptor than SARS-CoV RBD in comparison. S1 contains a receptor-binding domain (RBD) that can specifically bind to angiotensin-converting enzyme 2 (ACE2), the receptor on target cells.

Specifications

• For Use With (Application): Control, ELISA Standard, Binding Assay
• Format: Lyophilized
• Formulation: PBS with 5% mannitol, 5% trehalose, 0.01% Tween 80 and no preservative; pH 7.4
• Molecular Weight (g/mol): 25.1 kDa
• Name: SARS-CoV-2 Spike Protein (RBD)
• pH Range: 7.4
• Purification Method: Purified
• Quantity: 100 μg
• Storage Requirements: -20°C, Avoid Freeze/Thaw Cycles
• Regulatory Status: RUO
• Endotoxin Concentration: <1.0 EU/μg
• Gene Alias: Coronavirus; COVID; Covid 19; COVID19; COVID-19; SARS; SARS Coronavirus; SARS CoV; SARS CoV2; SARS CoV-2; SARS Spike Protein; SARS-CoV2; SARS-CoV-2
• Common Name: SARS-CoV-2 Spike Protein RBD
• Biological Activity: Measured by its binding ability in a functional ELISA. Immobilized S3-2019-nCov-RBD (at 2 μg/mL (100 μL/well) can bind ACE2 Protein, Human, Recombinant (mFc Tag) , the EC50 of ACE2 Protein, Human, Recombinant (mFc Tag) is 20-80 ng/mL)
• Product Type: Protein
• Conjugate: Unconjugated
• Recombinant: Recombinant
• Sequence: SARS-CoV-2 spike RBD, amino acids Arg319-Phe541 (Accession # YP_009724390.1)
• Content And Storage: -20°C, Avoid Freeze/Thaw Cycles
• Activity: Measured by its binding ability in a functional ELISA. Immobilized S3-2019-nCov-RBD at 2 μg/mL (100 μL/well) can bind ACE2 Protein, Human, Recombinant (mFc Tag) , the EC50 of ACE2 Protein, Human, Recombinant (mFc Tag) is 20-80 ng/mL.
• Expression System: HEK293 cells
• Form: Lyophilized
• Purity or Quality Grade: ≥90% as determined by SDS-PAGE.