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ACROBiosystems ACROBiosystems SARS-CoV-2 Spike Trimer (K417N, D614G), His Tag (MALS verified)

Supplier:  ACROBiosystems SPNC52HM50UG

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Recombinant Protein;50UG;SARS-CoV-2 S protein (K417N,D614G), His Tag (SPN-C52Hm) is expressed from human 293 cells (HEK293). It contains AA Val 16 - Pro 1213 (Accession # QHD43416.1). The mutations (K417N, D614G) were identified in the SARS-CoV-2 variant (known as B.1.351 or 20C/501Y.V2) which emerged in South Africa. Proline substitutions (F817P, A892P, A899P, A942P, K986P, V987P) and alanine substitutions (R683A and R685A) are introduced to stabilize the trimeric prefusion state of SARS-CoV-2 S protein and abolish the furin cleavage site, respectively.;This protein carries a polyhistidine tag at the C-terminus. The protein has a calculated MW of 138.0 kDa. The protein migrates as 160-200 kDa under reducing (R) condition (SDS-PAGE) due to glycosylation.;Immobilized SARS-CoV-2 Spike Trimer , His Tag (Cat. No. SPN-C52Hm) at 1 μg/mL (100 μL/well) can bind Human ACE2, Fc Tag (Cat. No. AC2-H5257) with a linear range of 3.1-25 ng/mL (QC tested).;Immobilized Human ACE2, Fc Tag (Cat. No. AC2-

Catalog No. 50-210-7134


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