Invitrogen™ Mouse SDF-1 alpha/CXCL12 ELISA Kit

Catalog No.	Quantity
EMCXCL12X5	5 x 96 Tests
EMCXCL12	96 Tests
EMCXCL12X10	10 x 96 Tests

Catalog No. EMCXCL12X5 Supplier Invitrogen™ Supplier No. EMCXCL12X5

Description

ELISA

The Mouse SDF-1 alpha/CXCL12 ELISA quantitates Ms SDF-1α in mouse serum, plasma, or cell culture medium. The assay will exclusively recognize both natural and recombinant Ms SDF-1α. Principle of the method The Mouse SDF-1α solid-phase sandwich ELISA (enzyme-linked immunosorbent assay) is designed to measure the amount of the target bound between a matched antibody pair. A target-specific antibody has been pre-coated in the wells of the supplied microplate. Samples, standards, or controls are then added into these wells and bind to the immobilized (capture) antibody. The sandwich is formed by the addition of the second (detector) antibody, a substrate solution is added that reacts with the enzyme-antibody-target complex to produce measurable signal. The intensity of this signal is directly proportional to the concentration of target present in the original specimen. Rigorous validation Each manufactured lot of this ELISA kit is quality tested for criteria such as sensitivity, specificity, precision, and lot-to-lot consistency. See manual for more information on validation.

CXCL12 is a stromal cell-derived alpha chemokine member of the intercrine family. This gene product and its receptor CXCR4 can activate lymphocytes and have been implicated in the metastasis of some cancers such as breast cancer. Mutations in this gene are associated with resistance to human immunodeficiency virus type 1 infections. Multiple transcript variants encoding different isoforms have been found for this gene.

Specifications

• Accession Number: P40224
• Assay Range: 3-600 pg/mL
• Assay Sensitivity: 3 pg/mL
• Calibration Range: 3 pg/mL to 600 pg/mL
• Description: SDF-1 alpha CXCL12 ELISA Kit, 96 Assays, 1 Kit, Mouse Species, Streptavidin-HRP Conjugate, Colorimetric Detection Method, Enzyme-Linked Immunosorbent Assay Designed for the Quantitation and Detection of SDF-1 alpha CXCL12 in Samples Using a Microplate Rea
• Detectable Analytes: SDF-1 alpha CXCL12, mouse SDF-1
• Assay: Solid-phase sandwich ELISA
• Conjugate: HRP
• Detection Method: Colorimetric
• Immunoassay Kit Format: Sandwich ELISA Kit
• Product Type: ELISA
• Protein Family: Chemokines & Receptors
• Sample Type: Plasma, Serum, Supernatant
• Specificity: This ELISA kit shows no cross-reactivity with any of the cytokines tested: Mouse CD30, L CD30, T CD40, CRG-2, CTACK, CXCL16, Eotaxin, Eotaxin-2, Fas Ligand, Fractalkine, GCSF, GM-CFS, IFN-gamma, IGFBP-3, IGFBP-5, IGFBP-6, IL-1 alpha, IL-1 beta, IL-2, IL-3
• Sufficient For: 5 x 96 assays
• Target Species: Mouse
• For Use With (Application): ELISA, Protein Assays, Protein Biology, Cancer Biology, Immunology, Neuroscience, Neurobiology, Protein Detection, Protein Analysis
• For Use With (Equipment): Absorbance Microplate Reader
• Gene Alias: 12-O-tetradecanoylphorbol 13-acetate repressed protein 1; AI174028; C Cmotif chemokine; C X C motif chemokine; CC motif chemokine; CCmotif chemokine; chemokine (C-X-C motif) ligand 12; chemokine (C-X-C motif) ligand 12 (stromal cell-derived factor 1); CXC; CXC motif chemokine; C-X-C motif chemokine 12; C-X-C motif chemokine ligand 12; CXCL; Cxcl12; hIRH; hSDF-1; Intercrine reduced in hepatomas; IRH; PBSF; pre-B cell growth-stimulating factor; pre-B-cell growth-stimulating factor; Scyb12; Sdf1; SDF-1; SDF-1 gamma; SDF1A; SDF-1a; SDF-1-alpha(3-67); SDF1B; SDF-1b; SDF-1-beta(3-72); Stromal cell-derived factor 1; stromal cell-derived factor-1 alpha; stromal cell-derived factor-1 gamma; thymic lymphoma cell-stimulating factor; TLSF; Tlsfa; TLSF-a; Tlsfb; TLSF-b; Tpar112-O-tetradecanoylphorbol 13-acetate repressed protein 1; AI174028; C Cmotif chemokine; C X C motif chemokine; CC motif chemokine; CCmotif chemokine; chemokine (C-X-C motif) ligand 12; chemokine (C-X-C motif) ligand 12 (stromal cell-derived factor 1); CXC; CXC motif chemokine; C-X-C motif chemokine 12; C-X-C motif chemokine ligand 12; CXCL; Cxcl12; hIRH; hSDF-1; Intercrine reduced in hepatomas; IRH; PBSF; pre-B cell growth-stimulating factor; pre-B-cell growth-stimulating factor; Scyb12; Sdf1; SDF-1; SDF-1 gamma; SDF1A; SDF-1a; SDF-1-alpha(3-67); SDF1B; SDF-1b; SDF-1-beta(3-72); Stromal cell-derived factor 1; stromal cell-derived factor-1 alpha; stromal cell-derived factor-1 gamma; thymic lymphoma cell-stimulating factor; TLSF; Tlsfa; TLSF-a; Tlsfb; TLSF-b; Tpar1; Pbsf; Scyb12; Sdf1; Tlsf; Tpar1
• Gene ID (Entrez): 20315
• Gene Symbol: CXCL12
• Host Species: Mouse
• Interassay CV: <12%
• Intraassay CV: <10%
• Kit Contents: Pre-coated 96 well plate, Standard, Assay Diluent concentrate, Biotinylated Detection Antibody, SAV-HRP, Wash Buffer, Chromogen, Stop Solution, Adhesive Plate Covers, Standard, Assay Diluent concentrate, Biotinylated Detection Antibody, SAV-HRP, Wash Buffer, Chromogen, Stop Solution, Adhesive Plate Covers
• Label or Dye: Biotin
• Quantity: 5 x 96 Tests
• Regulatory Status: RUO
• Research Discipline: Immunology
• Sample Volume: Plasma 50 μL, Serum 50 μL, Supernatant 100 μL
• Shipping Condition: Wet or Dry Ice
• Storage Requirements: Maintain refrigerated at 2-8°C for up to 6 months. For long term storage store at -20°C
• Synonym: Pbsf, Scyb12, Sdf1, Tlsf, Tpar1
• Target: CXCL12
• Test Time: 1 hr 20 min
• Total Assay Time: 4 hr 45 min