The ClonExpress Ultra One Step Cloning Kit V3 uses optimized technology for highly efficient homologous recombination of 1 to 11 DNA fragments. It features a newly optimized 2 × CE Mix V3, which ensures a high cloning success rate even with complicated situations like large vectors and GC-rich/poor homologous arms. It also enhances compatibility with varying amounts of DNA input, addressing issues of reduced cloning efficiency due to low DNA fragment concentration.
- Robust performance: assembles 1 to 11 fragments with a positive rate exceeding 95%.
- High efficiency: rapid recombination in 5 to 30 min, leading to high clone numbers and positivity rates.
- Special optimization: compatible with 15 to 35 bp overlaps, not just 15 to 20 bp.
- Broad compatibility: suitable for ultra-low DNA input, GC-rich/poor overlaps, and low-purity samples.