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AnaSpec SENSOLYTE 520 TEV ACTIVITY KIT

Supplier:  AnaSpec AS72227

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SensoLyte 520 TEV Activity Assay Kit *Fluorimetric*, (1 kit) Tobacco Etch Virus (TEV) proteinase is widely used to remove fusion tags from recombinant proteins. It recognizes specific sequence Glu-Asn-Leu-Tyr-Phe-Gln-Gly and cleaves between Glutamine and Glycine residues. TEV protease is active even at 4 Deg.C, making it the protease of choice for fusion proteins or tags removal. The SensoLyte TEV Protease Assay Kit uses TEV specific Glu-Asn-Leu-Tyr-Phe-Gln-Gly substrate labeled with 5-FAM dye and QXL 520 quencher. Proteolytic cleavage of this quenched 5-FAM-QXL 520 conjugate yields a brightly green fluorescence, which can be continuously monitored at Ex/Em= 490/520 nm. Increase in fluorescence intensity is directly proportional to the TEV protease quantity/activity.

Catalog No. NC0939635


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