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AnaSpec SENSOLYTE GREEN PROTEASE KIT
Supplier: AnaSpec AS71124
SensoLyte Green Protease Assay Kit *Fluorimetric*, (1 kit) The SensoLyte Green Protease Assay Kit is widely used for detection of generic protease activities. The kit uses a casein derivative that is heavily labeled with a rhodamine derivative, resulting in almost total quenching of the conjugate's fluorescence. Protease-catalyzed hydrolysis relieves this quenching conjugate, yielding brightly green fluorescent dye-labeled peptides. The increase in fluorescence intensity is directly proportional to protease activity (Ex/Em=488/520 nm). The SensoLyte protease assay kits do not require any separation steps and can be used to continuously measure the kinetics of a variety of exopeptidases and endopeptidases. The kit contains: Fluorescent labeled casein with high ratio of dye/protein (pH-insensitive) Trypsin (as positive control) Assay buffer A detailed protocol
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