Sephadex(R) LH20 is a liquid chromatography media for molecular sizing of natural product such as steroid terpenoid lipid low molecular weight peptide. Useful to analytical and industrial scale for preparation of closely related molecular species.Sephadex(R) LH-20 is prepared by hydroxypropylation of Sephadex(R) G-25 a bead-formed dextran medium and has been specifically developed for gel filtration of natural products such as steroids terpenoids lipids and low molecular weight peptides in organic solvents.Sephadex(R) LH-20 is useful at both analytical and industrial scale for the preparation of closely related molecular species. Due to the physico-chemical properties of this media it can be used either during initial purification prior to polishing by high performance ion exchange or reversed phase chromatography or as the final polishing step e.g. during the preparation of diastereomers. Sephadex(R) LH-20 is characterized by (1) Chromatographic selectivity due to dual hydro