Sigma Aldrich Fine Chemicals Biosciences SEQPLEX I WGA Kit Whole Ge

Supplier:  Sigma Aldrich Fine Chemicals Biosciences SEQXI96RXN

The SeqPlex-i WGA kit allows amplification of small quantities of DNA or from degraded/highly fragmented DNA for direct input onto Illumina(R) next-generation sequencing (NGS) flow cells. The SeqPlex-i process is comprised of three steps Pre-amplification/Library Synthesis Amplification 1 and Amplification 2.Step 1 In the Pre-amplification/Library Synthesis step (using the Library Preparation Reagents) the template DNA is replicated using primers comprised of semi-degenerate 3- ends with universal 5- ends. As polymerization proceeds displaced single strands serve as new templates for additional primer annealing and extension producing random overlapping replicons flanked by a universal primer (5) and primer complement (3) sequence.Step 2 In the Amplified Library Synthesis step (using the Amplification 1 Reagents) products from pre-amplification/library synthesis are amplified by single primer PCR via the universal end sequence. These amplification products typically range from 200

Catalog No. 50-291-4676