Sigma Aldrich Fine Chemicals Biosciences SEQPLEX I WTA Kit Whole Tr

Supplier:  Sigma Aldrich Fine Chemicals Biosciences SEQRI

The SeqPlex-I WTA kit allows amplification of small quantities of reverse transcribed RNA or degraded RNA for direct input onto Illumina(R) next-generation sequencing (NGS) flow cells. The SeqPlex-i process is comprised of three steps Pre-amplification/Library Synthesis Amplification 1 and Amplification 2Step 1 In the Pre-amplification/Library Synthesis step using the (Library Preparation Reagents) the template RNA is reverse transcribed using primers composed of a semi-degenerate 3- and universal 5-ends. As polymerization proceeds displaced and RNaseH generated single strands serve as new templates for additional primer annealing and extension producing random overlapping cDNAs flanked by a universal primer (5) and primer complement (3) sequence.Step 2 In the Amplified Library Synthesis step (using the Amplification 1 Reagents) products from pre-amplification/library synthesis are amplified by single primer PCR via the universal end sequence. These amplification products typicall

Catalog No. 50-290-9063