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Rockland Immunochemicals Sheep Hemagglutination Kit
Supplier: Rockland Immunochemicals KPA3913
10X Phosphate Buffered Saline (PBS) is a concentrated stock solution and should be diluted appropriately with distilled, deionized water or equivalent to its final working concentration which is 1X for this kit.SHEEP RED BLOOD CELLS (RBC) 10% Washed Pooled Cells - R405-0005 should be diluted in 1X PBS to 0.05% concentration.Anti-SHEEP Red Blood Cell (RBC) (RABBIT) Antibody - 213-4139-0002 binds and thus hemagglutinate Sheep RBCs when used at a dilution range of 1:25-1:50. An antibody mediated hemagglutination control is useful as a qualitative index for the integrity of the RBCs in the assay. Further serial dilutions up to 1:400 can be included in the assay if an independent or antibody-based control is required in the assay.
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