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ACROBiosystems ACROBiosystems Human Siglec-15 / CD33L3 Protein, Fc Tag

Supplier:  ACROBiosystems SG5H5253100UG

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Recombinant Protein;100UG;Human Siglec-15, Fc Tag (SG5-H5253) is expressed from human 293 cells (HEK293). It contains AA Phe 20 - Thr 263 (Accession # Q6ZMC9-1).;This protein carries a human IgG1 Fc tag at the C-terminus. The protein has a calculated MW of 53.0 kDa. The protein migrates as 55-60 kDa under reducing (R) condition (SDS-PAGE) due to glycosylation.;Immobilized Human Siglec-15, Fc Tag (Cat. No. SG5-H5253) at 5 μg/mL (100 μL/well) can bind Mouse Human chimeric MAb (5G12, Human IgG1) with a linear range of 0.4-6 ng/mL (QC tested).;Immobilized Human Siglec-15, Fc Tag (Cat. No. SG5-H5253) at 10 μg/mL (100 μL/well) on Diamond Protein A Protein, His Tag precoated (0.5 μg/well) plate, can bind Neu5Ac(a2-6)GalNAc-PAA-biotin with a linear range of 0.078-1.25 μg/mL (Routinely tested).;Immobilized Neu5Ac(a2-6)GalNAc-PAA-biotin on SA Chip can bind Human Siglec-15, Fc Tag (Cat. No. SG5-H5253) with an affinity constant of 4.49 μM as determined in a SPR assay (Biacore T200) (Routinely test

Catalog No. 50-195-7030


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