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ACROBiosystems ACROBiosystems Human Siglec-2 / CD22 Protein, His Tag

Catalog No. 501957346
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Recombinant Protein;1MG;Human Siglec-2, His Tag (CD2-H52H8) is expressed from human 293 cells (HEK293). It contains AA Asp 20 - Arg 687 (Accession # P20273-1).;This protein carries a polyhistidine tag at the C-terminus. The protein has a calculated MW of 77.0 kDa. The protein migrates as 90-116 kDa under reducing (R) condition (SDS-PAGE) due to glycosylation.;Immobilized Anti-Human CD22 Mab at 2 μg/mL (100 μL/well) can bind Human Siglec-2, His Tag (Cat. No. CD2-H52H8) with a linear range of 20-156 ng/mL (QC tested).;293 cells were transfected with anti-CD22-scFv and RFP tag. 2e5 of the cells were first stained with B. Human Siglec-2, His Tag (Cat. No. CD2-H52H8, 3 μg/mL) and C. His Tag Protein Control, followed by FITC Anti-6xHis Tag Antibody. A. Non-transfected 293 cells and C. His Tag Protein Control were used as negative control. RFP was used to evaluate CAR (anti-CD22-scFv) expression and FITC was used to evaluate the binding activity of Human Siglec-2, His Tag (Cat. No. CD2-H52H8)

Catalog No. 50-195-7346 Supplier ACROBiosystems Supplier No. CD2H52H81MG
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Recombinant Protein;1MG;Human Siglec-2, His Tag (CD2-H52H8) is expressed from human 293 cells (HEK293). It contains AA Asp 20 - Arg 687 (Accession # P20273-1).;This protein carries a polyhistidine tag at the C-terminus. The protein has a calculated MW of 77.0 kDa. The protein migrates as 90-116 kDa under reducing (R) condition (SDS-PAGE) due to glycosylation.;Immobilized Anti-Human CD22 Mab at 2 μg/mL (100 μL/well) can bind Human Siglec-2, His Tag (Cat. No. CD2-H52H8) with a linear range of 20-156 ng/mL (QC tested).;293 cells were transfected with anti-CD22-scFv and RFP tag. 2e5 of the cells were first stained with B. Human Siglec-2, His Tag (Cat. No. CD2-H52H8, 3 μg/mL) and C. His Tag Protein Control, followed by FITC Anti-6xHis Tag Antibody. A. Non-transfected 293 cells and C. His Tag Protein Control were used as negative control. RFP was used to evaluate CAR (anti-CD22-scFv) expression and FITC was used to evaluate the binding activity of Human Siglec-2, His Tag (Cat. No. CD2-H52H8)

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