Invitrogen™ Human SIK1, GST Tag Recombinant Protein

Catalog No.	Quantity
PV6446	100 μg

Catalog No. PV6446 Supplier Invitrogen™ Supplier No. PV6446

Description

Recombinant Protein

For maximum recovery please spin prior to use. Unless noted below, aliquots of the 5 μg, 10 μg and 20 μg sizes of kinase are not recommended as materials can be used in original packaging until exhausted. For larger sizes, the number of freeze/thaws may be reduced by preparing aliquots, aliquots below 20 μL are not recommended. Please never store a kinase diluted. If properly stored at -80C, this product is guaranteed for 6 months from date of purchase. This recombinant human SIK1 (1-303) was expressed by baculovirus in Sf9 insect cells using an N-terminal GST tag. Validated in LanthaScreen™ Kinase Binding Assay Protocols available for simple use and optimized performance Learn more about our kinase proteins > Related Links:LanthaScreen™ Kinase Activity AssayLanthaScreen™ Eu Kinase Binding Assay Protein Form: Recombinant, Active, Catalytic The gene accession number for SIK1 is NP_775490.2.

SIK1 may mediate the physiological effects of LKB1, including its tumor suppressor function. SIK1 is also involved in signaling by various proteins like STRAD, NUAK1, NUAK2, BRSK1, BRSK2, QIK, QSK, SIK, MARK1, MARK2, MARK3, MARK4, and MELK that are related to AMPK. Activation of SIK1 by phosphorylation on thr322 can lead to an increase in the catalytic activity of sodium/potassium ATPase alpha subunit at the plasma membrane. This results in an increase in intracellular sodium in intact mammalian cells. The family has been classified in 8 major groups based on sequence comparison of their tyrosine (PTK) or serine/threonine (STK) kinase catalytic domains. The STE group (homologs of yeast Sterile 7, 11, 20 kinases) consists of 50 kinases related to the mitogen-activated protein kinase (MAPK) cascade families (Ste7/MAP2K, Ste11/MAP3K, and Ste20/MAP4K). MAP kinase cascades, consisting of a MAPK and one or more upstream regulatory kinases (MAPKKs) have been best characterized in the yeast pheromone response pathway. Pheromones bind to Ste cell surface receptors and activate yeast MAPK pathway.

Specifications

• Accession Number: P57059
• Concentration: See Label
• For Use With (Application): Kinase Assay
• Formulation: 50mM tris HCl with 150mM NaCl, 10mM glutathione, 0.25mM DTT, 25% glycerol, 0.1mM PMSF, 0.1mM EDTA and no preservative; pH 7.5
• Gene ID (Entrez): 150094
• Molecular Weight (g/mol): 61 kDa
• Name: Human SIK1, GST Tag
• pH Range: 7.5
• Purification Method: Purified
• Quantity: 100 μg
• Storage Requirements: -80°C, Avoid Freeze/Thaw Cycles
• Regulatory Status: RUO
• Gene Alias: EIEE30; HRT-20; Kid2; MSK; Myocardial SNF1-like kinase; Probable serine/threonine protein kinase SNF1LK (HRT-20) (Myocardial SNF1-like kinase); protein kinase KID2; salt inducible kinase 1; salt-inducible kinase 1; salt-inducible protein kinase 1; serine/threonine protein kinase; serine/threonine-protein kinase SIK1; Serine/threonine-protein kinase SNF1-like kinase 1; serine/threonine-protein kinase SNF1LK; SIK; Sik1; SIK-1; SNF1-like kinase; SNF1LK
• Common Name: SIK1
• Gene Symbol: SIK1
• Protein Length: 1-303
• Product Type: Protein
• Conjugate: Unconjugated
• Species: Human
• Recombinant: Recombinant
• Protein Tag: GST-tag
• Sequence: 1-303
• Content And Storage: -80°C, Avoid Freeze/Thaw Cycles
• Kinase Group: Serine/Threonine Kinase
• Expression System: Baculovirus
• Protein Family: Kinases & Inhibitors
• Protein Form: Recombinant, Catalytic Domain
• Form: Liquid
• Research Category: Drug Development, Signal Transduction