Invitrogen™ Human SIK3, GST Tag Recombinant Protein

Catalog No.	Quantity
PV6403	10 μg
PV6404	100 μg

Catalog No. PV6403 Supplier Invitrogen™ Supplier No. PV6403

Description

Recombinant Protein

For maximum recovery please spin prior to use. Unless noted below, aliquots of the 5 μg, 10 μg and 20 μg sizes of kinase are not recommended as materials can be used in original packaging until exhausted. For larger sizes, the number of freeze/thaws may be reduced by preparing aliquots, aliquots below 20 μL are not recommended. Please never store a kinase diluted. If properly stored at -80C, this product is guaranteed for 6 months from date of purchase. Recombinant human SIK3 (1-307) was expressed by baculovirus in Sf9 insect cells using an N-terminal GST tag. Validated in LanthaScreen™ Kinase Binding Assay Protocols available for simple use and optimized performance Multiple pack sizes available for convenient use Related Product:LanthaScreen™ Eu Kinase Binding Assay Protein Form: Recombinant.

SIK3 (QSK) is a serine/threonine-protein kinase, belongs to QIK subfamily. The phosphorylation of SIK3 by LKB1 through the 14-3-3 binding enhances its catalytic activity and leads its localization to punctate structures within the cytoplasm. Overexpression of SIK3 promotes G1/S cell cycle progression with ovarian cancer. There are two sites (H331L and A1103V) were mutated at significant frequency in breast cancer. SIK3 is a novel tumor-associated antigen (TAA). SIK3 is a positive regulator of mTOR signaling that functions by triggering the degradation of DEPTOR, an mTOR inhibitor. Involved in the dynamic regulation of mTOR signaling in chondrocyte differentiation during skeletogenesis. Negatively regulates cAMP signaling pathway possibly by acting on CRTC2/TORC2 and CRTC3/TORC3.

Specifications

• Accession Number: Q9Y2K2
• Concentration: See Label
• For Use With (Application): Kinase Assay
• Formulation: 50mM tris HCl with 0.1mM PMSF, 25% glycerol, 0.1mM EDTA, 150mM NaCl, 10mM glutathione, 0.25mM DTT and no preservative; pH 7.5
• Gene ID (Entrez): 23387
• Molecular Weight (g/mol): 61.7 kDa
• Name: Human SIK3, GST Tag
• pH Range: 7.5
• Purification Method: Purified
• Quantity: 10 μg
• Storage Requirements: -80°C, Avoid Freeze/Thaw Cycles
• Regulatory Status: RUO
• Gene Alias: 5730525O22Rik; 9030204A07Rik; AI447252; AI846254; AI849445; Kiaa0999; L19; mKIAA0999; QSK; Salt-inducible kinase 3; serine/threonine-protein kinase QSK; Serine/threonine-protein kinase SIK3; SIK family kinase 3; SIK3; SIK-3
• Common Name: SIK3
• Gene Symbol: SIK3
• Protein Length: 59-365
• Product Type: Protein
• Conjugate: Unconjugated
• Species: Human
• Recombinant: Recombinant
• Protein Tag: GST-tag
• Sequence: 59-365
• Content And Storage: -80°C, Avoid Freeze/Thaw Cycles
• Assay Entry: Biochemical binding/kinetic
• Kinase Group: Serine/Threonine Kinase
• Target Entry: SIK3
• Expression System: Baculovirus
• Protein Family: Kinases & Inhibitors
• Protein Form: Recombinant, Fragment
• Form: Liquid
• Protein Subtype: Serine/Threonine Kinases