Gibco™ SILAC RPMI 1640 Flex Media, no glucose, no phenol red

Catalog No.	Quantity
A2494201	500 mL

Catalog No. A2494201 Supplier Gibco™ Supplier No. A2494201

Description

SILAC RPMI 1640 Flex Media, no glucose, no phenol red

SILAC RPMI 1640 Medium is RPMI 1640 basal cell culture medium without L-arginine, L-glutamine, and L-lysine for use during SILAC protein labeling with stable isotope-labeled lysine and/or arginine. RPMI 1640 Medium is widely used for supporting the growth of many different mammalian cells. Cells successfully cultured in RPMI-1640 include HeLa, Jurkat, MCF-7, PC12, PBMC, astrocytes, and carcinomas. We offer a variety of Gibco™ RPMI 1640 modifications for a range of applications. Find the right modification using the media selector tool.

RPMI 1640 Medium is unique from other media because it contains the reducing agent glutathione and high concentrations of vitamins. RPMI 1640 Medium contains biotin, vitamin B12, and PABA, which are not found in Eagle's Minimal Essential Medium or Dulbecco's Modified Eagle Medium. In addition, the vitamins inositol and choline are present in very high concentrations. RPMI 1640 Medium contains no proteins, lipids, or growth factors. Therefore, RPMI 1640 Medium requires supplementation, commonly with 10% Fetal Bovine Serum (FBS). SILAC protein labeling experiments should be conducted using Gibco™ Dialyzed FBS. RPMI 1640 Medium uses a sodium bicarbonate buffer system (2.0 g/L), and therefore requires a 5-10% CO₂ environment to maintain physiological pH.

cGMP Manufacturing and Quality System
SILAC RPMI 1640 Medium is manufactured at a cGMP-compliant facility located in Grand Island, New York. The facility is registered with the FDA as a medical device manufacturer and is certified to ISO 13485 standards.

Specifications

• Cell Type: Mammalian Cells
• Form: Liquid
• Product Type: RPMI 1640
• Serum Level: Standard Serum Supplementation
• Without Additives: No Glucose, No Glutamine, No HEPES, No Phenol Red, No Sodium Pyruvate, No Arginine, No Lysine
• Product Line: Gibco
• Quantity: 500 mL
• Content And Storage: Store in refrigerator (2–8°C) and protect from light.
  Shelf life: 12 months from date of manufacture.
• Culture Type: Mammalian Cell Culture

For Research Use or Further Manufacturing. Not for use in diagnostic procedures.