ATCC SIMA9 BRAIN MICROGLIA MOUSE MU

Supplier:  ATCC CRL3265

SIM-A9 is a microglial cell line that was derived from cerebral cortex tissues collected and pooled from mouse pups. The tissues were dissociated by trypsinization and plated. On day 14 the microglial cells were harvested by vigorously shaking the flasks and the plating out detached microglial cells. The plates were maintained for 2 weeks at which time an unexpected, extensive proliferation of microglial cells was observed. The cells were passaged 7 times over a 4-week period and were determined to have become spontaneously immortalized. The cells were then plated out in a limited dilution procedure and colonies derived from wells observed to contain a single cell were expanded. A clone designated as “A-9” gave rise to this cell line. These spontaneously immortalized micgoglial cells exhibit phagocytic activity upon liposaccharide stimulation and TNFα cytokine secretion upon β-amyloid exposure. These cells may be used in place of primary microglial cultures, whic

Catalog No. 50-238-3461