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Cytoskeleton, Inc. SiR700-Actin Kit
Supplier: Cytoskeleton, Inc. CYSC013
Composed of: 35 nmol SiR700-Actin and 1 µmol verapamil. SiR700-actin is based on the far-red silicon rhodamine (SiR) fluorophore analogue SiR700 and the actin binding natural product jasplakinolide. SiR700-actin allows labeling of endogenous F-actin in live cells with high specificity and low background without the need for genetic manipulation or over-expression. The key features of SiR700-actin are i) far-red absorption and emission wavelengths, ii) cell permeability, iii) fluorogenic character, and iv) compatibility with super-resolution microscopy (e.g., STED & SIM). In addition, SiR700 actin can be used for wide-field and confocal fluorescent imaging in living cells. The emission in the far-red wavelength minimizes phototoxicity and sample autofluorescence. SiR700-actin is compatible with GFP and/or m-cherry fluorescent proteins.
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