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ILEX LIFE SCIENCES LLC Biocolor Sircol S2000 Fragmentation Reagent, Insoluble Collagen Assay Component (3 x 110 ml)
Supplier: ILEX LIFE SCIENCES LLC SFRAG
The Biocolor Sircol™ INSOLUBLE Collagen Assay is a dye-binding method for the analysis of insoluble collagen fibers. The colorimetric assay uses the Sircol™ Dye Reagent, which contains Sirius Red dye in picric acid. The assay can be used to assess the rate of production of newly laid down collagen fibers during periods of rapid growth, development, tissue repair, remodeling and wound healing. Sources of material includes tissues, bone and calcified tissue. It replaces the conventional hydroxyproline analysis. No more working with concentrated acids or alkalis! This assay uses mild acid and temperature treatment for 2 to 3 hrs. The solubility conversion to denatured collagen and subsequent measurement can be completed within one working day. The ratio of soluble and insoluble collagens can be measured using the same Sircol™ Dye Reagent. Sensitivity: 10 µg. Assay Run Time: 4 hours.
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