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Eurofins Discoverx PathHunter SIRPa Signaling Bioassay Kit, 2pl. (93-1135Y19-00129)
Supplier: Eurofins Discoverx 931135Y1900129
The PathHunter SIRPa Signaling Bioassay consists of human cells engineered to measure drug potency and detect neutralizing antibodies. Engagement of the receptor by its ligand, or agonist antibodies results in phosphorylation of the receptor’s cytosolic tail. The SH2-domain fused to EA binds the phosphorylated receptor, forcing complementation of ED and EA, resulting in formation of an active β-gal enzyme, which hydrolyzes the substrate to generate a chemiluminescent signal. The bioassay kit contains all reagents needed for the assay including pre-qualified cryopreserved cells, detection reagents, cell plating reagent, positive control agonist, and assay plates. For detailed product information, User manual and COA, visit www.discoverx.com/bioassay.
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