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ACROBiosystems ACROBiosystems PE-Labeled Human SIRP alpha / CD172a Protein, Fc Tag (recommended for neutralizing assay)
Supplier: ACROBiosystems SIAHP252250TESTS
Recombinant Protein;250TESTS;PE-Labeled Human SIRP alpha, Fc Tag (recommended for neutralizing assay) (Cat. No. SIA-HP252) is produced via conjugation of PE to Human SIRP alpha, Fc tag under optimal conditions. Human SIRP alpha, Fc tag is expressed in HEK293 cells containing AA Glu 31 - Arg 370 (Accession # P78324-1).;This protein carries a human IgG1 Fc tag at the C-terminus. The protein has a calculated MW of 65.7 kDa. ;Flow Cytometry assay shows that PE-Labeled Human SIRP alpha, Fc Tag (recommended for neutralizing assay) (Cat. No. SIA-HP252) can bind to 293T cells overexpressing human CD47. 1 μl stock solution per million cells (QC tested).;FACS analysis shows that the binding of PE-Labeled Human SIRP alpha, Fc Tag (recommended for neutralizing assay) (Cat. No. SIA-HP252) to 293T overexpressing CD47 was inhibited by increasing concentration of neutralizing anti-human CD47 antibody. The IC50 is 0.37 μg/ml (QC tested).
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